BURDENIUKBACTERIAL CONTAMINATION OF THE INTACT KIDNEY IN COURSE OF THE ACUTE UNILATERAL KIDNEY INFLAMMATION IN RATS: DIFFERENT TIME FRAMES AFTER NEPHRECTOMY ON THE AFFECTED SIDE

M.S. STEPANCHENKO, O.S. FEDORUK,, I.P. BURDENIUK,, O. V. BLINDER,, V.I. Burdeniuk

Abstract


Summary. Exceptionally clinically important is clear understanding of the pathophysiological mechanisms for localization and migration of pathologic microorganisms towards the intact kidney in course of the unilateral inflammatory process. The study aimed to determine the intact kidney’s pathogenic species composition and numerosity in course of rat acute unilateral kidney inflammation, different time frames after the affected organ removal. The experiment was carried out on 54 mature nonlinear white rats Rattus Norwegicus. In all animals an acute unilateral kidney inflammation has been modeled. For modeling of the acute unilateral kidney inflammation in experimental rats, an E.Coli strain was used. The pathogen was previously isolated from urine in patients with the diagnosed urinary tract infection. In order to determine the affected kidney influence on the intact organ microbial contamination, nephrectomy on the affected side was performed on the 10th, 14th and 21st days. Bacterial concentrations in the residual kidney tissue were determined 4, 7 and 14 days after nephrectomy. Control group constituted animals without nephrectomy. Scores were compared to the respective ones of the intact kidney in control group, on the same day of experiment. Reliable changes of almost all microbiologic contamination scores were detected after removal of the affected organ. Infection of the inoculated kidney spread onto intact contralateral organ by the hematogenous and/or lymphogenous routes and persisted there for some specific time. The nature of the infection in the contralateral kidney changed between the 10th and 14th days after inoculation – the agent apparently penetrated the kidney tissue and developed inflammation. Removal of the inoculated kidney timely, before the infection of the intact organ has progressed to its invasive stage, facilitated complete elimination of the pathogen in the contralateral kidney by the 28th day.

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